Réaction #2146728

Équation de réaction

glucose

ammonium sulfate

KH2PO4

VB1

Biotin

KOH

calcium carbonate

→

biotin

L-glutamic acid

Conditions de réaction

Traitement

1. 1
   AutreThe single cross-over recombinant thus obtained
2. 2
   AutreThe wild-type 13869 strain and 2A-1 strain were inoculated in 20 ml of a flask
3. 3
   AutreAfter the sugar was completely consumed
4. 4
   workup.ADDITIONdiluted to 101 times

Mode opératoire

The single cross-over recombinant thus obtained was named 2A-1 strain. The wild-type 13869 strain and 2A-1 strain were inoculated in 20 ml of a flask medium (30 g/l glucose, 15 g/l ammonium sulfate, 1 g/l KH2PO4, 0.4 g/l MgSO4.7H2O, 0.01 g/l FeSO4.7H2O, 0.01 g/l MnSO4.4-5H2O, 200 μg/l VB1 (vitamin B1), 300 μg/l Biotin, and 0.48 g/l soybean hydrolysates (T-N: total nitrogen), adjusted to pH 8.0 with KOH: autoclaved at 115° C. for 10 minutes), followed by addition of 1 g of heat-sterilized calcium carbonate, and each of the strains was cultured with shaking at 31.5° C. After the sugar was completely consumed, the concentration of L-glutamic acid which had accumulated in the medium was determined. The results are shown in Table 1 (OD620 is turbidity at 620 nm of culture solution diluted to 101 times, and indicates the cell amount, and Glu (g/L) indicates the amount of accumulated L-glutamic acid). It was found that the 2A-1 strain produced L-glutamic acid in the presence of an excess amount of biotin, whereas the parent strain ATTCC 13869 did not produce L-glutamic acid at all.

Source