Réaction #2028936

Équation de réaction

EDC

N-[(1S)-1-methyl-2-phenylethyl]butane-1,4-diamine

di-isopropylethylamine

→

Amphetamine

Solvants

water

dioxan

water

water

Conditions de réaction

Traitement

1. 1
   workup.STIRRINGThe mixture was shaken for 18 hours at 20-25° C
2. 2
   AutreThe above material was dialysed against 50 mM Tris buffer at pH 7.4
3. 3
   workup.ADDITIONcontaining sodium azide using a 10,000 molecular weight
4. 4
   AutreBCA protein assay after dialysis
5. 5
   Autregave a concentration of 1.6 mg/ml

Mode opératoire

To BSA (24.8 mg) in water (4 ml) was added NHS (22.2 mg, 0.193 mmol) in water (0.5 ml) followed by EDC (41 mg, 0.199 mmol) in water (0.5 ml). The mixture was shaken for 15 minutes at 20-25° C. N-[(1S)-1-methyl-2-phenylethyl]butane-1,4-diamine (2.7 mg, 0.013 mmol) in dioxan (0.2 ml) followed by di-isopropylethylamine (0.02 ml). The mixture was shaken for 18 hours at 20-25° C. The above material was dialysed against 50 mM Tris buffer at pH 7.4 containing sodium azide using a 10,000 molecular weight cut off membrane. BCA protein assay after dialysis gave a concentration of 1.6 mg/ml.

Source