Réaction #2028934

ord-cd89d2d4c51b43b5bc9a2ae30c4c9ecd

Équation de réaction

CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC(O)CO.CCCCO.OCCO
U-2000
→
NCCCC[C@H](N)C(=O)O
L-Lysine

Réactifs

CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
—
polyoxyethylene sorbitan monooleic acid ester
CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC(O)CO.CCCCO.OCCO
U-2000

Réactifs

Aucun

Solvants

OCC(O)CO
glycerol

Conditions de réaction

Conditions détaillées
See reaction.notes.procedure_details.

Traitement

  1. 1
    Autrethe strain being obtained in of Example 9
  2. 2
    workup.ADDITIONThe obtained suspension containing the bacteria
  3. 3
    Autreequipped with baffles such that the turbidity
  4. 4
    Autreat 37° C.
  5. 5
    Autrefor 48 hours

Mode opératoire

A glycerol stock of the strain in which fadIJ operon was amplified, the strain being obtained in of Example 9, was thawed. 100 μL of each was evenly spread on an LB agar medium plate containing 50 mg/L ampicillin and 25 mg/L streptomycin and incubated at 37° C. for 24 hours. About ¼ volume of bacterial cells on the plate was suspended in 1.0 mL of saline and turbidity thereof at a wavelength of 600 nm was measured by Spectrophotometer U-2000 (Hitachi). The obtained suspension containing the bacteria was inoculated into 40 mL of fermentation medium described below containing 50 mg/L ampicillin and 25 mg/L streptomycin in a 500 mL-Erlenmeyer flask equipped with baffles such that the turbidity thereof at a wavelength of 600 nm was 0.25; and cultured using a rotary shaking culture apparatus at a stirring rate of 200 rpm at 37° C. for 48 hours. As a carbon source in a main culture, sodium oleate added with polyoxyethylene sorbitan monooleic acid ester (Tween 80: manufactured by NACALAI TESQUE, INC.) as an emulsification promoter to a final concentration of 0.5% (w/v) was used. As for a total amount of carbon sources, sodium oleate was 10 g/L. The medium composition used for the culture is shown below.

Source

DOI: 10.6084/m9.figshare.5104873.v1Brevet: US08389249B2uspto-grants-2013_03