Réaction #2028932

ord-36c993148ffd4f798a56e6b6031afd3e

Équation de réaction

CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
→
NCCCC[C@H](N)C(=O)O
L-Lysine

Réactifs

CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
—
polyoxyethylene sorbitan monooleic acid ester
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin

Réactifs

Aucun

Solvants

OCC(O)CO
Glycerol

Conditions de réaction

Conditions détaillées
See reaction.notes.procedure_details.

Traitement

  1. 1
    Autreat 37° C.
  2. 2
    Autrefor 72 hours

Mode opératoire

Glycerol stocks of the strains obtained in Example 3, in which fad genes were amplified, were thawed. And, 100 μL of each was evenly spread on an LB agar medium plate containing 50 mg/L ampicillin and 20 mg/L streptomycin and incubated at 37° C. for 24 hours. About ½ volume of bacterial cells on the obtained plate were inoculated into 5 mL of fermentation medium described below containing 50 mg/L ampicillin and 20 mg/L streptomycin in a test tube manufactured by AGC TECHNO GLASS CO., LTD. (diameter×length×thickness (mm)=25×200×1.2), and cultured using a reciprocal shaking culture apparatus at 37° C. for 72 hours. As a carbon source in a main culture, sodium oleate added with polyoxyethylene sorbitan monooleic acid ester (Tween 80: manufactured by NACALAI TESQUE, INC.) as an emulsification promoter to a final concentration of 0.5% (w/v) was used. As for a total amount of carbon sources, sodium oleate was 10 g/L. The medium composition used for the culture is shown below.

Source

DOI: 10.6084/m9.figshare.5104873.v1Brevet: US08389249B2uspto-grants-2013_03