Réaction #1994313

Équation de réaction

DIPEA

Boc-Arg-OH

NH(CH2Ph)2

PyCloP

→

Boc-Arg-N(CH2Ph)2

Solvants

DMF

DCM

Conditions de réaction

Traitement

1. 1
   workup.ADDITIONwas added
2. 2
   workup.STIRRINGThe solution was stirred for 1 h at room temperature
3. 3
   AutreThe reaction mixture was evaporated
4. 4
   workup.DISSOLUTIONredissolved in ethyl acetate
5. 5
   Lavagewashed with citric acid, sodium bicarbonate and brine
6. 6
   AutreThe solvent was removed under vacuum

Mode opératoire

A solution of Boc-Arg-OH(1 eq), NH(CH2Ph)2 (1.1 eq) and PyCloP (1 eq) in dry DCM (filtered through alumina) (2 ml) and DMF (1 ml). The solution was cooled on ice and DIPEA (2 eq) was added under stirring. The solution was stirred for 1 h at room temperature. The reaction mixture was evaporated, and redissolved in ethyl acetate and washed with citric acid, sodium bicarbonate and brine. The solvent was removed under vacuum and the Boc protecting group of the resulting peptide was deprotected in the dark using 95% TFA.

Source