Réaction #1269837

ord-2178096666c84924908923167b0ca2c0

Équation de réaction

FC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)Br
Perfluorooctyl bromide
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](N)C[C@@H]1O.O=S(=O)(O)O
Tobramycin sulfate
CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCCCC
DSPC
O.O.[Ca+2].[Cl-].[Cl-]
calcium chloride dihydrate
→
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](N)C[C@@H]1O
Tobramycin

Réactifs

FC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)Br
Perfluorooctyl bromide
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](N)C[C@@H]1O.O=S(=O)(O)O
Tobramycin sulfate
CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCCCC
DSPC
O.O.[Ca+2].[Cl-].[Cl-]
calcium chloride dihydrate
—
phosphatidylcholine
—
PFOB-in-water

Réactifs

Aucun

Solvants

O
water
O
Water

Conditions de réaction

Conditions détaillées
See reaction.notes.procedure_details.

Traitement

  1. 1
    SéchageA tobramycin sulfate dry powder composition
  2. 2
    Autreis prepared
  3. 3
    workup.ADDITIONThe resulting lipid dispersion is mixed in an UltraTurrax T-50 (IKA Labortechnik) at 8,000 rpm for 5 min
  4. 4
    workup.ADDITIONunder mixing
  5. 5
    workup.ADDITIONAfter the addition
  6. 6
    workup.ADDITIONis mixed for an additional 10 min at 10,000 rpm
  7. 7
    AutreEmulsification in the UltraTurrax produces droplets in the micron-size range
  8. 8
    Autredrying
  9. 9
    AutreThe feedstock is then spray dried
  10. 10
    Autreto obtain a dry powder formulation

Mode opératoire

A tobramycin sulfate dry powder composition is prepared according to the following procedure. Sterile Water for Irrigation (SWFI) is heated above the gel to liquid crystal temperature (about 80° C.) of disteroyl phosphatidylcholine (DSPC). DSPC and calcium chloride dihydrate are then added to the heated water. The resulting lipid dispersion is mixed in an UltraTurrax T-50 (IKA Labortechnik) at 8,000 rpm for 5 min. Perfluorooctyl bromide (PFOB) is then added dropwise (15 ml/min) to the lipid dispersion under mixing. After the addition is complete the resulting PFOB-in-water emulsion is mixed for an additional 10 min at 10,000 rpm. Emulsification in the UltraTurrax produces droplets in the micron-size range. Tobramycin sulfate is then dissolved in the continuous phase of the emulsion and the resulting dispersion is used as the feedstock for spray drying. The feedstock is then spray dried to obtain a dry powder formulation having the composition set forth in Table 1 below.

Source

DOI: 10.6084/m9.figshare.5104873.v1Brevet: US08664187B2uspto-grants-2014_03