Reacción #939015

Ecuación de reacción

LTC4

LTA4 methyl ester

serine borate

→

Leukotriene C4

Disolventes

phosphate

Condiciones de reacción

Tratamiento posterior

1. 1
   Otrofor 30 minutes
2. 2
   Otroat 37° C
3. 3
   OtroThe reaction was terminated by addition of ice-cold methanol

Procedimiento

LTC4 synthase prepared as a crude fraction from guinea pig lung was used. The test compounds7were tested in duplicate at a concentration of 300 μg/ml. The test compound and/or vehicle was incubated with 12 μg enzyme, 0.3 μg LTA4 methyl ester, 0.2% (w/w) albumin (to stabilize the product) and 4.5 mM serine borate (to prevent conversion of LTC4 to LTD4) in phosphate buffer pH 7.8 for 30 minutes at 37° C. The reaction was terminated by addition of ice-cold methanol. Formation of LTC4 was quantitated by RIA (radioimmunoassay).

Fuente