Reacción #93281

ord-eb793162060e4d608a6c1a06068b387a

Ecuación de reacción

CCCn1c(=O)c2[nH]c(-c3ccc(OCC(=O)O)cc3)nc2n(CCC)c1=O
Compound 6d
CCCn1c(=O)c2[nH]c(-c3ccc(OCC(=O)O)cc3)nc2n(CCC)c1=O
8-(4'-Carboxymethyloxyphenyl)-1,3-dipropyl xanthine
O=C(O)C(CCC[C@@H]1SC[C@H]2[C@@H]1NC(=O)N2O)N1C(=O)CCC1=O
N-Hydroxysuccinimido-d-biotin
CO
methanol
CCOCC
ether
→
CCCn1c(=O)c2[nH]c(-c3ccc(OCC(=O)O)cc3)nc2n(CCC)c1=O.[NH-]CCNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
8-(4'-Carboxymethyloxyphenyl)-1,3-dipropylxanthine 2-(biotinylamino)ethylamide

Reactantes

CCCn1c(=O)c2[nH]c(-c3ccc(OCC(=O)O)cc3)nc2n(CCC)c1=O
Compound 6d
CCCn1c(=O)c2[nH]c(-c3ccc(OCC(=O)O)cc3)nc2n(CCC)c1=O
8-(4'-Carboxymethyloxyphenyl)-1,3-dipropyl xanthine
O=C(O)C(CCC[C@@H]1SC[C@H]2[C@@H]1NC(=O)N2O)N1C(=O)CCC1=O
N-Hydroxysuccinimido-d-biotin
CO
methanol
CCOCC
ether

Reactivos

Ninguno

Disolventes

CN(C)C=O
DMF

Condiciones de reacción

Condiciones detalladas
See reaction.notes.procedure_details.

Tratamiento posterior

  1. 1
    OtroA solution formed after several minutes
  2. 2
    OtroThe precipitate was collected
  3. 3
    Otrodried (yield 26.6 mg, 73%)

Procedimiento

Compound 6d (24.1 mg, 0.056 mmol) was suspended in 1 ml DMF. N-Hydroxysuccinimido-d-biotin (Sigma, 23.6 mg, 0.069 mmol) was added with stirring. A solution formed after several minutes, and a precipitate appeared soon thereafter. After one day methanol (1 ml) and ether were added. The precipitate was collected and dried (yield 26.6 mg, 73%).

Fuente

DOI: 10.6084/m9.figshare.5104873.v1Patente: US04612315uspto-grants-1986_09