Reacción #72965

Ecuación de reacción

p-toluenesulfonyl chloride

1-{[2-(ethoxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]methyl}cyclohexanol

m-chloroperoxybenzoic acid

ammonium hydroxide

m-CPBA

→

1-{[4-amino-2-(ethoxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]methyl}cyclohexanol

Disolventes

dichloromethane

Water

CHCl3

Condiciones de reacción

Tratamiento posterior

1. 1
   workup.ADDITIONwas added over 1 h until the starting material
2. 2
   Otrowas consumed
3. 3
   workup.ADDITIONWater (40 mL) and solid K2CO3 were added to the reaction mixture until the pH=10
4. 4
   workup.ADDITIONThe mixture was poured into a separatory funnel
5. 5
   Extracciónextracted with chloroform
6. 6
   Otroresulting in an emulsion
7. 7
   ExtracciónThe aqueous layer was extracted with chloroform three times
8. 8
   LavadoThe combined organic layers were washed with water and brine
9. 9
   Secadodried over MgSO4
10. 10
    Filtraciónfiltered
11. 11
    Concentraciónconcentrated
12. 12
    Otroto yield a crude white solid (1.51 g)
13. 13
    Extracciónthe aqueous layer was extracted with dichloromethane (2×30 mL)
14. 14
    LavadoThe combined organic layers were washed with brine (25 mL)
15. 15
    Secadowere dried over MgSO4
16. 16
    Filtraciónfiltered
17. 17
    Concentraciónconcentrated
18. 18
    OtroThe crude product was purified by flash chromatography (silica gel, gradient elution with 98:1:1 CH2Cl2/MeOH/NH4OH to 94:5:1 CH2Cl2/MeOH/NH4OH)
19. 19
    Otrothen crystallized from acetonitrile

Procedimiento

To a solution of 1-{[2-(ethoxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]methyl}cyclohexanol (1.90 g, 5.60 mmol) in CHCl3 (43 mL) was added m-chloroperoxybenzoic acid (m-CPBA, 77% w/w, 1.26 g, 5.60 mmol) over 15 min. The reaction was monitored by thin layer chromatography (TLC) and more m-CPBA was added over 1 h until the starting material was consumed. Water (40 mL) and solid K2CO3 were added to the reaction mixture until the pH=10. The mixture was poured into a separatory funnel and extracted with chloroform, resulting in an emulsion. The aqueous layer was extracted with chloroform three times. The combined organic layers were washed with water and brine, dried over MgSO4, filtered, and concentrated to yield a crude white solid (1.51 g). The white solid (1.51 g, 4.24 mmol) was dissolved in dichloromethane (25 mL) at rt and concentrated ammonium hydroxide (16 mL) was added, followed by p-toluenesulfonyl chloride (TsCl, 0.81 g, 4.24 mmol). The mixture was stirred 1 day (d). Water (25 mL) was added and the aqueous layer was extracted with dichloromethane (2×30 mL). The combined organic layers were washed with brine (25 mL) and were dried over MgSO4, filtered, and concentrated. The crude product was purified by flash chromatography (silica gel, gradient elution with 98:1:1 CH2Cl2/MeOH/NH4OH to 94:5:1 CH2Cl2/MeOH/NH4OH) then crystallized from acetonitrile to yield 1-{[4-amino-2-(ethoxymethyl)-1H-imidazo[4,5-c]quinolin-1-yl]methyl}cyclohexanol as pale orange crystals that were dried under high vacuum (0.32 g, 16% over two steps), mp 186-188° C. 1H NMR (300 MHz, CDCl3) δ 8.15 (dm, J=8.4 Hz, 1H), 7.81 (dm, J=8.4 Hz, 1H), 7.52 (m, 1H), 7.32 (m, 1H), 5.38 (br s, 2H), 4.90 (br s, 2H), 4.74 (br s, 2H), 3.66 (q, J=7.0 Hz, 2H), 3.00 (s, 1H), 1.71-1.52 (m, 10), 1.25 (t, J=7.0 Hz, 3H). MS (APCI) m/z 355 (M+H+). Anal. calcd for C20H26O2N4: C, 67.77; H, 7.39; N, 15.81. Found: C, 67.52; H, 7.57; N, 15.78

Fuente