Reacción #674286

Ecuación de reacción

calcium chloride

hydrogen phosphate

NaOH

→

Calcium Phosphate

Condiciones de reacción

Tratamiento posterior

1. 1
   OtroCaP NPs
2. 2
   Otrowere obtained by filtration of the suspension through a 0.22 μm
3. 3
   Filtraciónfilter
4. 4
   OtroCaP NPs
5. 5
   Otrowere stored at 4° C. before use
6. 6
   OtroCaP/ZOL-NPs complexes (CaPZ NPs) were prepared
7. 7
   workup.ADDITIONby mixing a CaP NPs dispersion with an aqueous solution of ZOL
8. 8
   Concentraciónat different ZOL concentrations (10 mg/ml of ZOL in water or 50 mg/ml of ZOL in phosphate buffer at pH 9.5)

Procedimiento

An aqueous solution of calcium chloride (18 mM) was added, dropwise and under magnetic stirring, to an aqueous solution on dibasic hydrogen phosphate (10.8 mM). The pH of both solutions was adjusted beforehand to 9.5 with NaOH. CaP NPs were obtained by filtration of the suspension through a 0.22 μm filter. CaP NPs were stored at 4° C. before use. CaP/ZOL-NPs complexes (CaPZ NPs) were prepared by mixing a CaP NPs dispersion with an aqueous solution of ZOL at different ZOL concentrations (10 mg/ml of ZOL in water or 50 mg/ml of ZOL in phosphate buffer at pH 9.5), at a volume ratio of 50:1.

Fuente