Reacción #565514

Ecuación de reacción

chloroacetaldoxime

triethylamine

6-(2,4-dichlorophenyl)-8-ethyl-9H-purine

3-cyclopropyl-1-propyn-3-ol

chloroacetaldoxime

acetaldoxime

N-chlorosuccinimide

→

9-[1-Cyclopropyl-1-(3-methyl-isoxazol-5-yl)methyl]-6-(2,4-dichlorophenyl)-8-ethyl-9H-purine

Disolventes

DMF

methylene chloride

Condiciones de reacción

Tratamiento posterior

1. 1
   Extracciónextracting the product into diethyl ether
2. 2
   Lavadowashing with water
3. 3
   workup.ADDITION) The cyclo addition reaction
4. 4
   workup.ADDITIONwere added until all the starting material
5. 5
   Otrowas consumed
6. 6
   OtroThe reaction mixture was purified
7. 7
   workup.ADDITIONby adding directly to a column
8. 8
   Lavadoeluting
9. 9
   Otro72 mg of a white foam was collected

Procedimiento

To a stirring solution of the compound of Example 7241 (90 mg, 0.24 mmol; prepared in a manner similar to that of Example 2 using 6-(2,4-dichlorophenyl)-8-ethyl-9H-purine and 3-cyclopropyl-1-propyn-3-ol) in methylene chloride (2 mL) were added chloroacetaldoxime (25 mg, 0.27 mmol) and triethylamine (0.038 mL, 0.27 mmol). (The chloroacetaldoxime used was previously prepared by reacting equimolar amounts of acetaldoxime and N-chlorosuccinimide in DMF, then extracting the product into diethyl ether and washing with water.) The cyclo addition reaction was monitored by TLC and additional amounts of chloroacetaldoxime and triethylamine were added until all the starting material was consumed. The reaction mixture was purified by adding directly to a column packed with silica gel and eluting using a gradient of 100% hexane to 25% ethyl acetate in hexane. 72 mg of a white foam was collected. MS (NH3—CI) 428 (M+H+) HRMS: m/e=428.1037 (M+H+, C21H20Cl2N5O). Purity by reverse phase HPLC >97%.

Fuente