Reacción #543561

Ecuación de reacción

maltose

maltotriose

→

maltose

isomaltosyl maltose

Disolventes

acetate

Condiciones de reacción

Tratamiento posterior

1. 1
   OtroThe activity of α-isomaltosylglucosaccharide-forming enzyme
2. 2
   Otroto give a concentration of 2% (w/v) for a substrate solution
3. 3
   Otroat 35° C.
4. 4
   Otrofor 60 min
5. 5
   Otrofor 10 min
6. 6
   Otrothe enzymatic reaction

Procedimiento

The activities of these enzymes were assayed as follows: The activity of α-isomaltosylglucosaccharide-forming enzyme was assayed by dissolving maltotriose in 100 mM acetate buffer (pH 6.0) to give a concentration of 2% (w/v) for a substrate solution, adding 0.5 ml of an enzyme solution to 0.5 ml of the substrate solution, enzymatically reacting the mixture solution at 35° C. for 60 min, boiling the reaction mixture for 10 min to suspend the enzymatic reaction, and quantifying maltose, among the isomaltosyl maltose and maltose formed in the reaction mixture, on HPLC as described in Experiment 1. One unit activity of the α-isomaltosylglucosaccharide-forming enzyme was defined as the enzyme amount that forms one micromole of maltose per minute under the above enzymatic reaction conditions.

Fuente