Reacción #518099

ord-3c314d8a96834c93b9dc8b09d3f343b6

Ecuación de reacción

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O
NHS-LC-Biotin
CN(C)C=O
DMF
→
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
Biotin

Reactantes

—
Product #21335
O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O
NHS-LC-Biotin
CN(C)C=O
DMF

Reactivos

Ninguno

Condiciones de reacción

Condiciones detalladas
See reaction.notes.procedure_details.

Tratamiento posterior

  1. 1
    OtroPurified antibodies or their fragments
  2. 2
    Otroobtained
  3. 3
    Otrostored at 0-4° C.

Procedimiento

Purified antibodies or their fragments, obtained as described in paragraph A at 1 mg/ml in PBS, were biotinylated for 3 hours at 25° C. (ambient temperature) by adding 37 μl of 10 mg/ml solution of NHS-LC-Biotin, Product #21335; Pierce, Rockford, Ill) in anhydrous DMF. Subsequently, the derivatized antibody was buffer-exchanged on a G25F column into PBS and stored at 0-4° C.

Fuente

DOI: 10.6084/m9.figshare.5104873.v1Patente: US06656744B2uspto-grants-2003_12