Reacción #50906

Ecuación de reacción

biotinyl N-hydroxysuccinimide ester

→

biotin

Disolventes

sodium hydrogen carbonate

Condiciones de reacción

Tratamiento posterior

1. 1
   OtroThe reaction mixture was dialyzed overnight against 5 L of PBS at 4° C.

Procedimiento

Each 3-mg portion of the 32-clone monoclonal antibodies prepared in (1) was mixed with 10 mg of biotinyl N-hydroxysuccinimide ester (product of Zymed) and the reaction was carried out in 0.1 M sodium hydrogen carbonate (pH 8) with stirring at room temperature for 3 hours. The reaction mixture was dialyzed overnight against 5 L of PBS at 4° C. to give the biotin-labeled monoclonal antibody.

Fuente