Reacción #423211

ord-252de01952f54d03bdc6ddb409cdb7fa

Ecuación de reacción

c1ccc(CNc2ncc3[nH]cnc3n2)cc1
benzylaminopurin
OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O
saccharose
CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@@]3(O)C(=O)C[C@@H](C)O[C@H]3O[C@@H]2[C@H]1O.Cl.Cl
spectinomycin dihydrochloride
O=C(O)Cc1cccc2ccccc12
1-naphtalene acetic acid
→
CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@@]3(O)C(=O)C[C@@H](C)O[C@H]3O[C@@H]2[C@H]1O
Spectinomycin

Reactantes

c1ccc(CNc2ncc3[nH]cnc3n2)cc1
benzylaminopurin
OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O
saccharose
—
agarose
CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@@]3(O)C(=O)C[C@@H](C)O[C@H]3O[C@@H]2[C@H]1O.Cl.Cl
spectinomycin dihydrochloride
O=C(O)Cc1cccc2ccccc12
1-naphtalene acetic acid

Reactivos

Ninguno

Condiciones de reacción

Condiciones detalladas
See reaction.notes.procedure_details.

Tratamiento posterior

  1. 1
    ConcentraciónIt was found that the optimal concentration of selection

Procedimiento

When calli are about 0.5 mm in size they are transferred to callus growth medium SH2 (Schenk & Hildebrandt, Can. J. Bot. 50:199-204 (1972)) with 30 g/l saccharose, 5 g/l agarose, 0.1 mg/l 1-naphtalene acetic acid (NAA) and 0.1 mg/l benzylaminopurin (BAP), and the selecting agent spectinomycin dihydrochloride at concentrations of 0-1000 mg/l. It was found that the optimal concentration of selection is 500 mg/l. The non-resistant calli appear as white calli. They also grow slower as compared to the control calli. The culture conditions are as described above for the above protoplast calli.

Fuente

DOI: 10.6084/m9.figshare.5104873.v1Patente: US08884099B2uspto-grants-2014_11