Reacción #41107

Ecuación de reacción

2-aminoadamantane hydrochloride

DIPEA

TSTU

2′-Methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid

Intermediate 8

2′-Methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid

→

2′-methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid adamantan-2-ylamide

Rendimiento 77.0%

Disolventes

water

dichloromethane

DMF

Condiciones de reacción

Tratamiento posterior

1. 1
   Otrothe organic layer was separated
2. 2
   ExtracciónThe aqueous layer was extracted twice with dichloromethane
3. 3
   OtroThe combined organic phases were dried under vacuum
4. 4
   Otropurified by C-18

Procedimiento

2′-Methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid (Intermediate 8, 50 mg, 0.26 mmol) was dissolved in a mixture of dry dichloromethane (3.2 mL) and dry DMF (0.8 mL). DIPEA (0.23 mL, 1.3 mmol) and TSTU (93 mg, 0.28 mmol) were added to the above mixture. After the mixture was stirred for 1 h, the appearance of active ester was detected by LC-MS. Then 2-aminoadamantane hydrochloride (58 mg, 0.31 mmol) was added. After another 2 hours water was added and the organic layer was separated. The aqueous layer was extracted twice with dichloromethane. The combined organic phases were dried under vacuum and purified by C-18 reversed phase prep-HPLC with a gradient of 10-100% acetonitrile/water to give 2′-methyl-2′H-[1,3′]bipyrazolyl-4′-carboxylic acid adamantan-2-ylamide (65 mg, 77%). Mass spectrum: m/z: 362.2 (M+1).

Fuente