Reacción #40272

Ecuación de reacción

2-isobutyl-succinonitrile

KOH

water

→

potassium (S)-3-cyano-5-methylhexanoate

Rendimiento 31.3%

Condiciones de reacción

Tratamiento posterior

1. 1
   Otroreaction vessels
2. 2
   Otrothe product mixtures were decanted
3. 3
   Otroleaving the enzyme catalyst
4. 4
   Otroin the reaction vessels
5. 5
   OtroReaction buffer (20 mL)
6. 6
   workup.ADDITIONwas added to the each reaction vessel
7. 7
   workup.STIRRINGstirred for approximately 2 min.
8. 8
   Otrodecanted
9. 9
   workup.ADDITIONadded to the product mixtures
10. 10
    workup.ADDITIONby adding
11. 11
    Otro2-isobutyl-succinonitrile (6.81 g) and reaction buffer (118.2 mL) to each reaction vessel
12. 12
    workup.STIRRINGstirring the reaction
13. 13
    workup.WAITmixtures for 24 h
14. 14
    OtroAfter four reactions were completed in each vessel
15. 15
    Extracciónextracted with MTBE (3×500 mL)
16. 16
    OtroThe MTBE extracts were removed
17. 17
    Extracciónextracted with MTBE (2×500 mL)
18. 18
    ExtracciónThe MTBE extract of the acidified aqueous part
19. 19
    Concentraciónwas concentrated under vacuum
20. 20
    Otroto leave an oil, which
21. 21
    ConcentraciónThe resulting solution was concentrated under vacuum

Procedimiento

Two 125 mL jacketed reaction vessels maintained at 30° C. were each charged with 2-isobutyl-succinonitrile (6.81 g), NIT-102 C2 (1.70 g) and 118.2 mL of reaction buffer. After stirring for 24 h, the product mixtures were decanted, leaving the enzyme catalyst in the reaction vessels. Reaction buffer (20 mL) was added to the each reaction vessel, stirred for approximately 2 min., and then decanted and added to the product mixtures. Reactions were repeated by adding 2-isobutyl-succinonitrile (6.81 g) and reaction buffer (118.2 mL) to each reaction vessel and stirring the reaction mixtures for 24 h. After four reactions were completed in each vessel (total of eight batch reactions), the product mixtures were combined and extracted with MTBE (3×500 mL). The MTBE extracts were removed and the aqueous part adjusted to pH 2.1 with phosphoric acid and extracted with MTBE (2×500 mL). The MTBE extract of the acidified aqueous part was concentrated under vacuum to leave an oil, which was treated with water (100 mL) and KOH (8.5 g). The resulting solution was concentrated under vacuum to give 24.2 g (31.3%) of potassium (S)-3-cyano-5-methylhexanoate. Methyl (S)-3-cyano-5-methylhexanoate was prepared from potassium (S)-3-cyano-5-methylhexanoate and analyzed by chiral GC to reveal an enantiomeric purity of 99.1% e.e.

Fuente