Reacción #310432

ord-509c63b7c80e462a862f4e786f5ff757

Ecuación de reacción

Cl.NC(CO)(CO)CO
Tris-HCl
NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
NADP
→
NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
NADPH

Reactantes

—
sepharose-alcohol
Cl.NC(CO)(CO)CO
Tris-HCl
NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
NADP

Reactivos

Ninguno

Disolventes

CC(C)O
isopropanol

Condiciones de reacción

Temperatura
45°CELSIUS
Condiciones detalladas
See reaction.notes.procedure_details.

Tratamiento posterior

  1. 1
    Otrowas then immersed in a water bath
  2. 2
    Otrothe treated solution was collected in a cooled ethanol precipitation vessel
  3. 3
    workup.ADDITIONSeveral milliliters of petroleum ether were added to 20 ml of the eluted solution
  4. 4
    Otrothe precipitate was collected by centrifugation

Procedimiento

The gel was used to prepare a sepharose-alcohol dehydrogenase column (0.4×4.0 cm) which was then immersed in a water bath maintained at 45° C. A 0.01 M Tris-HCl solution (pH 9.0) containing 2.5 mg/ml NADP and 0.25 v/v isopropanol was applied to the column at a rate of 0.25 ml/min.; the treated solution was collected in a cooled ethanol precipitation vessel. Several milliliters of petroleum ether were added to 20 ml of the eluted solution and the precipitate was collected by centrifugation.

Fuente

DOI: 10.6084/m9.figshare.5104873.v1Patente: US04352885uspto-grants-1982_10