Reacción #2175509

ord-cd1d6bb629e64a15a977719921a72373

Ecuación de reacción

O.O.OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
trehalose dihydrate
CC(C[*:1])[*:2]
polypropylene
Cl
HCl
→
OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
Trehalose

Reactantes

O.O.OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
trehalose dihydrate
CC(C[*:1])[*:2]
polypropylene
Cl
HCl

Reactivos

Ninguno

Disolventes

O
water
O
water

Condiciones de reacción

Condiciones detalladas
See reaction.notes.procedure_details.

Tratamiento posterior

  1. 1
    workup.ADDITIONwas added at room temperature
  2. 2
    Otrowas obtained
  3. 3
    Otroto obtain 400 ml final volume
  4. 4
    FiltraciónThe solution was filtered through a 0.22-micron
  5. 5
    Filtraciónfilter
  6. 6
    workup.ADDITIONto dilute the protein solution

Procedimiento

25.48 g of trehalose dihydrate was carefully weighed and transferred into a sterile polypropylene bottle, to which 350 ml of purified water was added at room temperature and stirred slowly until a clear solution was obtained. To the clear solution, 0.1 N HCl was added drop by drop to adjust the pH to 3.9, then the volume was adjusted with purified water to obtain 400 ml final volume. The pH was measured and found to be 4.2. The solution was filtered through a 0.22-micron filter and was used directly to dilute the protein solution.

Fuente

DOI: 10.6084/m9.figshare.5104873.v1Patente: US08435943B2uspto-grants-2013_05