Reacción #2053072

Ecuación de reacción

H-Val-Gly-OBzl•HCl

Z-Glu-OBzl

Triethylamine

HOBt

WSC•HCl

→

Z-Glu(Val-Gly-OBzl)-OBzl

Disolventes

methylene chloride

Condiciones de reacción

Tratamiento posterior

1. 1
   Otrowas kept at 0° C
2. 2
   ConcentraciónThe reaction mixture was concentrated under reduced pressure
3. 3
   workup.DISSOLUTIONthe residue was dissolved in heated ethyl acetate (1500 ml)
4. 4
   LavadoThe solution was washed with water (200 ml), 5% citric acid aqueous solution (200 ml× twice), saturated brine (150 ml), 5% sodium hydrogencarbonate aqueous solution (200 ml× twice), and saturated brine (150 ml)
5. 5
   SecadoThe organic layer was dried over anhydrous magnesium sulfate
6. 6
   Otrothe magnesium sulfate was removed by filtration
7. 7
   Concentraciónthe filtrate was concentrated under reduced pressure
8. 8
   FiltraciónThe deposited crystals were collected by filtration
9. 9
   Otrodried under reduced pressure

Procedimiento

Then, H-Val-Gly-OBzl•HCl and Z-Glu-OBzl (5.57 g, 15.0 mmol) were dissolved in methylene chloride (50 ml), and the solution was kept at 0° C. Triethylamine (2.30 ml, 16.5 mmol), HOBt (1-hydroxybenzotriazole, 2.53 g, 16.5 mmol), and WSC•HCl (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, 3.16 g, 16.5 mmol) were added to the solution, and the mixture was stirred at room temperature for 2 days. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in heated ethyl acetate (1500 ml). The solution was washed with water (200 ml), 5% citric acid aqueous solution (200 ml× twice), saturated brine (150 ml), 5% sodium hydrogencarbonate aqueous solution (200 ml× twice), and saturated brine (150 ml). The organic layer was dried over anhydrous magnesium sulfate, then the magnesium sulfate was removed by filtration, and the filtrate was concentrated under reduced pressure. The deposited crystals were collected by filtration, and dried under reduced pressure to obtain white crystals of Z-Glu(Val-Gly-OBzl)-OBzl (6.51 g, 10.5 mmol).

Fuente