Reacción #2006447

Ecuación de reacción

7-methoxy-2-aminotetraline

sodium hydroxide

7-methoxy-2-aminotetraline

7-methoxy-2-aminotetraline

7-methoxy-2-tetralone

D-glucose

NAD+

phosphate

L-alanine

pyridoxal phosphate

7-methoxy-2-aminotetraline

→

(S)-7-methoxy-2-aminotetraline

Disolventes

water

Condiciones de reacción

Tratamiento posterior

1. 1
   workup.ADDITIONwere poured
2. 2
   workup.ADDITIONwas added so that total volume was 30 ml
3. 3
   Otroproduced in the reaction liquid
4. 4
   Otrowas produced

Procedimiento

Into a flask containing 300 mg of a substrate 7-methoxy-2-tetralone, 460 mg of D-glucose, 3 mg of NAD+, 3 ml of 1 M phosphate buffer solution (with a pH of 7), 910 mg of L-alanine, and 4.0 mg of pyridoxal phosphate, 6 ml of the bacterial suspension were poured, and then deionized water was added so that total volume was 30 ml. The resultant product was stirred at 30° C. for 25 hours while being adjusted to a pH of 6.8 by drippage of 5 N aqueous solution of sodium hydroxide. After the reaction, 7-methoxy-2-aminotetraline produced in the reaction liquid was analyzed under the following HPLC conditions, whereby the rate of conversion and optical purity were measured. In the result, it was found that 7-methoxy-2-aminotetraline was produced, but in such a small amount that the rate of conversion into 7-methoxy-2-aminotetraline was 1.5%. The absolute configuration of 7-methoxy-2-aminotetraline was (S), and the optical purity was 74.1% e.e.

Fuente