Reacción #1869223

Ecuación de reacción

acetonitrile

NaOH

Methyl 1-ethyl-6-(4-methoxy-2-methylphenylamino)-8-methyl-4-oxo-1,4-dihydroquinolone-3-carboxylate

acetonitrile

→

1-Ethyl-8-methyl-6-(3-methylpyridin-2-ylamino)-4-oxo-1,4-dihydroquinolone-3-carboxylic acid

Disolventes

water

dioxane

Condiciones de reacción

Tratamiento posterior

1. 1
   OtroRemoval of the solvent in vacuo
2. 2
   Otroto purify the product
3. 3
   OtroThe pure product was isolated from the reaction solution by chromatography on an HPLC system
4. 4
   Otrorose to 95% over the course of 20 minutes

Procedimiento

Methyl 1-ethyl-6-(4-methoxy-2-methylphenylamino)-8-methyl-4-oxo-1,4-dihydroquinolone-3-carboxylate (30 mg) was dissolved in 5 ml of dioxane, 2.5 equivalents of a 1 N NaOH solution were added, and the mixture was heated at 60° C. for 4 h. Removal of the solvent in vacuo was followed by chromatography on an HPLC system to purify the product. The pure product was isolated from the reaction solution by chromatography on an HPLC system. This entailed use of a Merck Purospher-RP18 column and an acetonitrile:water mixture as eluent; the initial acetonitrile content was 15% and rose to 95% over the course of 20 minutes.

Fuente