Reacción #1747849

ord-34cbc220044e4ddfa08a0e8d4e75a805

Ecuación de reacción

CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN
desferrioxamine
CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN
desferrioxamine
O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21
fluorescein isothiocyanate
O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21
FITC
→
CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN.O=C(O)c1ccccc1-c1c2ccc(=O)cc-2oc2cc(O)ccc12
Fluorescein Desferrioxamine

Reactantes

CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN
desferrioxamine
CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN
desferrioxamine
O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21
fluorescein isothiocyanate
O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21
FITC

Reactivos

Ninguno

Disolventes

O=C([O-])[O-].[Na+].[Na+]
sodium carbonate
[Al]
aluminum

Condiciones de reacción

Condiciones detalladas
See reaction.notes.procedure_details.

Tratamiento posterior

  1. 1
    Otroto react for at least 24 hours at room temperature
  2. 2
    OtroTo separate fluorescently labeled desferrioxamine from free fluorescein and free desferrioxamine
  3. 3
    Otrothe mixture is separated
  4. 4
    Lavadoare eluted in 2 mL fractions
  5. 5
    LavadoThe fluorescein-desferrioxamine is eluted in the void volume of the column
  6. 6
    OtroThe first peak is collected
  7. 7
    Otroused for preparing fluorescein-desferrioxamine, desferrioxamine, and rhodamine liposomes

Procedimiento

An aliquot of 120 mg of desferrioxamine and 233.64 mg of fluorescein isothiocyanate (FITC) are mixed in 50 mL of 0.1 M sodium carbonate pH 9.5. A molar ratio of 3:1 of FITC: desferrioxamine is used as described in Hentz et al. (1997) Anal. Chem. 69:4994-5000. The reaction mixture is wrapped in aluminum foil to avoid light exposure and allowed to react for at least 24 hours at room temperature. To separate fluorescently labeled desferrioxamine from free fluorescein and free desferrioxamine, the mixture is separated using a P-2 column (Bio-Rad) equilibrated in saline and samples are eluted in 2 mL fractions. The fluorescein-desferrioxamine is eluted in the void volume of the column. The first peak is collected and used for preparing fluorescein-desferrioxamine, desferrioxamine, and rhodamine liposomes.

Fuente

DOI: 10.6084/m9.figshare.5104873.v1Patente: US08029795B2uspto-grants-2011_10