Reacción #1477379

Ecuación de reacción

L-histidine ammonia

urocanic acid

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L-histidine

Condiciones de reacción

Procedimiento

[L-histidine ammonia-lyase activity is indicated in terms of micromoles of urocanic acid which are produced by reaction with L-histidine. The reaction with L-histidine is conducted by adding 10 g of the immobilized preparation to 30 ml of an aqueous 0.25 M L-histidine solution (pH 9.0), and shaking the mixture at 37° C. for one hour. The amount of urocanic acid produced in assayed colorimetrically at 277 nm (molecular extinction coefficient = 1.88 × 10-4 (pH 7.4)).]

Fuente