Reacción #1375840

ord-a3562e6deb6a4016935eaf47602ff2ab

Ecuación de reacción

OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)CO
D-sorbitol
O=P([O-])([O-])[O-].[K+].[K+].[K+]
potassium phosphate
→
O=C(CO)[C@@H](O)[C@H](O)[C@@H](O)CO
L-sorbose

Reactantes

OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)CO
D-sorbitol
O=P([O-])([O-])[O-].[K+].[K+].[K+]
potassium phosphate

Reactivos

Ninguno

Disolventes

O
water

Condiciones de reacción

Condiciones detalladas
See reaction.notes.procedure_details.

Tratamiento posterior

  1. 1
    Otrowas incubated at 30° C. with gentle shaking

Procedimiento

A reaction mixture (total volume 1.04 ml) containing 0.2 ml of purified SLDH (0.04 mg protein), 0.04 ml of 0.2M PMS, 0.1 ml of 0.4M D-sorbitol, 0.4 ml of 0.5M potassium phosphate buffer (pH 7.0) and 0.3 ml of water was incubated at 30° C. with gentle shaking. As a result, L-sorbose was formed at the rate of about 1.3 mg/hour.

Fuente

DOI: 10.6084/m9.figshare.5104873.v1Patente: US05747301uspto-grants-1998_05