Reaction #992712

ord-8b49e00b6bdf4dafa5fb643bb52bd5ec

Reaction equation

O=c1ccn([C@H]2C[C@H](O)[C@@H](CO)O2)c(=O)[nH]1
2′-deoxyuridine
O=P([O-])([O-])[O-].[K+].[K+].[K+]
potassium phosphate
→
O=P([O-])(O)O.[K+]
KH2PO4
O=c1ccn([C@H]2C[C@H](O)[C@@H](CO)O2)c(=O)[nH]1
2-deoxyUridine

Reactants

O=c1ccn([C@H]2C[C@H](O)[C@@H](CO)O2)c(=O)[nH]1
2′-deoxyuridine
O=P([O-])([O-])[O-].[K+].[K+].[K+]
potassium phosphate

Reagents

None

Solvents

O
water

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

5.4 grams (equal to 32 mMoles) of 2-chloroadenine were weighed and suspended while being stirred in 50 ml of DMSO. Solubilisation occurred with the addition of 70 ml of 25% (w:v) KOH, obtaining a perfectly clear solution with a 266 mMolar concentration of 2-chloroAdenine. 14.6 grams (equal to 64 mMoles) of 2′-deoxyuridine and 4 grams (equal to 30 mMoles) of anhydrous monobasic potassium phosphate were dissolved in about 600 ml of deionised water, obtaining a 106 mM for the 2-deoxyUridine and 50 mM for KH2PO4. The pH was corrected to a value of 7.5 with 25% (w:v) KOH as required.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08012717B2uspto-grants-2011_09