Reaction #92668

ord-91d959a2855c4ca69e96db9f71cfe589

Reaction equation

CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2C3=C[C@@H]4OC45C[C@@H](O)CC[C@]5(C)[C@H]3CC[C@]12C
5,6α-epoxicholest-7-en-3β-ol
NCCCN
1,3-diaminopropane
CCCCO
1-Butanol
→
CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2C3=C[C@@H](NCCCN)[C@@]4(O)C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C
5α-Hydroxy-6β-(3-aminopropylamino)-cholest-7-en-3β-ol
Yield 66.0%

Reactants

CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2C3=C[C@@H]4OC45C[C@@H](O)CC[C@]5(C)[C@H]3CC[C@]12C
5,6α-epoxicholest-7-en-3β-ol
NCCCN
1,3-diaminopropane
CCCCO
1-Butanol

Reagents

None

Solvents

COC(C)(C)C
methyl-tertbutyl-ether

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    Otherequipped with a magnetic stirrer bar
  2. 2
    Temperaturethe mixture heated
  3. 3
    Temperatureto reflux for 40 h
  4. 4
    Washwashed with water (5 vol) and with brine (5 vol)
  5. 5
    Washeluted with methyl-tertbutyl-ether (3 vol)
  6. 6
    Otherthe solvent was removed under reduced pressure

Procedure

5,6α-epoxicholest-7-en-3β-ol (8.9 g, 22.1 mmol, 1 eq) and 1,3-diaminopropane (3.3 g, 44.1 mmol, 2 eq) were charged in a round-bottomed flask equipped with a magnetic stirrer bar. 1-Butanol (70 ml, 5 vol) was added and the mixture heated to reflux for 40 h. The reaction mixture was cooled at r.t., diluted with methyl-tertbutyl-ether (5 vol) and washed with water (5 vol) and with brine (5 vol). The organic layer was passed through a silica pad (40 g) eluted with methyl-tertbutyl-ether (3 vol) then 10% Methanol/ethyl acetate (60 vol). Fractions of interest were pooled and the solvent was removed under reduced pressure to give 5α-Hydroxy-6β-(3-aminopropylamino)-cholest-7-en-3β-ol as a white solid (6.9 g, 66%). In same conditions, use of ethanol as solvent (40 vol) and Ca(OTf)2 as catalyst gave a 21% yield.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US09447141B2uspto-grants-2016_09