Reaction #892

Reaction equation

DL-lysine monohydrochloride

L-lysine

→

D-lysine monohydrochloride

Conditions

Workup

1. 1
   OtherInto a shaking flask having
2. 2
   workup.ADDITIONa volume of 500 ml was charged ml of a medium (pH 7.0)
3. 3
   Extractionextract
4. 4
   workup.WAITcultured at 30° C. for 20 hours
5. 5
   OtherThe cell collected from 1000 ml of the above culture broth by centrifugation
6. 6
   Othercollected by centrifugation
7. 7
   workup.ADDITIONTo the cell was added 500 ml of a 50 mM phosphate buffer (pH 7.0)
8. 8
   workup.ADDITIONcontaining 50 g of DL-lysine monohydrochloride
9. 9
   Otherthe mixture was reacted at 30° C. for 72 hours
10. 10
    OtherAfter the reaction
11. 11
    Otherthe cells were removed by centrifugation, and subsequent procedures

Procedure

Into a shaking flask having a volume of 500 ml was charged ml of a medium (pH 7.0) comprising 0.5% of DL-lysine monohydrochloride, 1.0% of polypeptone, 1.0% of a yeast extract and 0.5% of sodium chloride, and the medium was sterilized at 120° C. for 10 minutes. A loopful of Pseudomonas sp. ATCC 14676 was inoculated into the medium and cultured at 30° C. for 20 hours. The cell collected from 1000 ml of the above culture broth by centrifugation was suspended in a physiological saline and then collected by centrifugation. To the cell was added 500 ml of a 50 mM phosphate buffer (pH 7.0) containing 50 g of DL-lysine monohydrochloride, and the mixture was reacted at 30° C. for 72 hours to degrade L-lysine completely. After the reaction, the cells were removed by centrifugation, and subsequent procedures were carried out in the same manner as in Example 1 to obtain 14.2 g of D-lysine monohydrochloride.

Source