Reaction #82860

ord-1825b608ebc244d3b221ee45bb66642a

Reaction equation

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

→

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA DOPC

Reactants

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

Reagents

None

Solvents

chloroform

Conditions

Detailed conditions

See reaction.notes.procedure_details.

Workup

1
OtherDOTMA/DOPC vesicles were prepared
2
Otherremoving the solvent
3
Otherby drying under a stream of nitrogen gas
4
Otherwas placed under vacuum overnight
5
Otherto remove traces of solvent
6
Othersonicated in a bath type sonicator for 30 minutes

Procedure

DOTMA/DOPC vesicles were prepared by dissolving 10 mg DOTMA and 10 mg DOPC in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 1 ml water with vigorous shaking and sonicated in a bath type sonicator for 30 minutes until clear producing DOTMA/DOPC SUV's at a concentration of 20 mg/ml total lipid.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05622712uspto-grants-1997_04