Reaction #674287

ord-9fb1ff1cb2d94f1da5086a474289cc48

Reaction equation

[Ca+2].[Cl-].[Cl-]
calcium chloride
O=P([O-])([O-])O
hydrogen phosphate
[Na+].[OH-]
NaOH
→
O=P([O-])([O-])[O-].O=P([O-])([O-])[O-].[Ca+2].[Ca+2].[Ca+2]
Calcium Phosphate

Reactants

[Ca+2].[Cl-].[Cl-]
calcium chloride
O=P([O-])([O-])O
hydrogen phosphate
[Na+].[OH-]
NaOH

Reagents

None

Solvents

O=P([O-])([O-])[O-]
phosphate

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    OtherCaP NPs
  2. 2
    Otherwere obtained by filtration of the suspension through a 0.22 μm
  3. 3
    Filtrationfilter
  4. 4
    OtherCaP NPs
  5. 5
    Otherwere stored at 4° C. before use
  6. 6
    OtherCaP/ZOL-NPs complexes (CaPZ NPs) were prepared

Procedure

An aqueous solution of calcium chloride (18 mM) was added, dropwise and under magnetic stirring, to an aqueous solution on dibasic hydrogen phosphate (10.8 mM). The pH of both solutions was adjusted beforehand to 9.5 with NaOH. CaP NPs were obtained by filtration of the suspension through a 0.22 μm filter. CaP NPs were stored at 4° C. before use. CaP/ZOL-NPs complexes (CaPZ NPs) were prepared by mixing a CaP NPs dispersion with an aqueous solution of (50 mg/ml of ZOL in phosphate buffer at pH 9.5), at a volume ratio of 50:1.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US09226897B2uspto-grants-2016_01