Reaction #674286

ord-f3d8a68c81474492834b414a03b1058d

Reaction equation

[Ca+2].[Cl-].[Cl-]
calcium chloride
O=P([O-])([O-])O
hydrogen phosphate
[Na+].[OH-]
NaOH
→
O=P([O-])([O-])[O-].O=P([O-])([O-])[O-].[Ca+2].[Ca+2].[Ca+2]
Calcium Phosphate

Reactants

[Ca+2].[Cl-].[Cl-]
calcium chloride
O=P([O-])([O-])O
hydrogen phosphate
[Na+].[OH-]
NaOH

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    OtherCaP NPs
  2. 2
    Otherwere obtained by filtration of the suspension through a 0.22 μm
  3. 3
    Filtrationfilter
  4. 4
    OtherCaP NPs
  5. 5
    Otherwere stored at 4° C. before use
  6. 6
    OtherCaP/ZOL-NPs complexes (CaPZ NPs) were prepared
  7. 7
    workup.ADDITIONby mixing a CaP NPs dispersion with an aqueous solution of ZOL
  8. 8
    Concentrationat different ZOL concentrations (10 mg/ml of ZOL in water or 50 mg/ml of ZOL in phosphate buffer at pH 9.5)

Procedure

An aqueous solution of calcium chloride (18 mM) was added, dropwise and under magnetic stirring, to an aqueous solution on dibasic hydrogen phosphate (10.8 mM). The pH of both solutions was adjusted beforehand to 9.5 with NaOH. CaP NPs were obtained by filtration of the suspension through a 0.22 μm filter. CaP NPs were stored at 4° C. before use. CaP/ZOL-NPs complexes (CaPZ NPs) were prepared by mixing a CaP NPs dispersion with an aqueous solution of ZOL at different ZOL concentrations (10 mg/ml of ZOL in water or 50 mg/ml of ZOL in phosphate buffer at pH 9.5), at a volume ratio of 50:1.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US09226897B2uspto-grants-2016_01