Reaction #6431

Reaction equation

hydrochloric acid

glutaric anhydride

N-hydroxysuccinimide

triethylamine

→

mono N-hydroxysuccinimide glutarate

Solvents

dichloromethane

Conditions

Workup

1. 1
   Extractionextracted with ethyl acetate

Procedure

One mole of glutaric anhydride and one mole of N-hydroxysuccinimide were treated in dichloromethane with 1.2 equivalents of triethylamine. This reaction was stirred for 40 minutes, acidified by the addition of 0.5N hydrochloric acid (HCl) (aqueous), and then extracted with ethyl acetate to form mono N-hydroxysuccinimide glutarate. This glutarate was then combined with 5 moles of thionyl chloride and stirred at room temperature for three hours. The resulting reaction mixture was dried to remove volatiles, and thereby form N-hydroxysuccinimidyl glutaryl chloride.

Source