Reaction #568884

ord-f7d06aaadbea43349f4cc150feb7e9ba

Reaction equation

[N-]=[N+]=[N-].[Na+]
sodium azide
O=P([O-])([O-])O.[Na+].[Na+]
disodium hydrogen orthophosphate
O=P([O-])(O)O.[K+]
potassium dihydrogen orthophosphate
Cl
HCl
[Cl-].[K+]
potassium chloride
→
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline

Reactants

—
sodium chloride NaCl
[N-]=[N+]=[N-].[Na+]
sodium azide
O=P([O-])([O-])O.[Na+].[Na+]
disodium hydrogen orthophosphate
O=P([O-])(O)O.[K+]
potassium dihydrogen orthophosphate
Cl
HCl
[Cl-].[K+]
potassium chloride
—
buffer solution

Reagents

None

Solvents

O
water
O
water

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    Otherto degas either in a screw-capped container overnight or under vacuum

Procedure

Dulbecco's phosphate buffered saline (DPBS) is prepared by first dissolving, sequentially, about 160 g sodium chloride NaCl), about 4 grams potassium chloride (KCl), about 23 grams disodium hydrogen orthophosphate (Na2HPO4), about 4 grams potassium dihydrogen orthophosphate (KH2PO4), and about 10 grams sodium azide in a liter of reverse-osmosis (MilliQ) water. Then, the pH is adjusted to about 7.3 by adding appropriate amounts of HCl. Finally, the buffer solution is diluted to 1:20 (50 ml buffer solution with 950 ml reverse-osmosis water), and allowed to degas either in a screw-capped container overnight or under vacuum.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05789461uspto-grants-1998_08