Reaction #511640
ord-535ac1a8fe8f44c28e3893af634bb01b
Reaction equation
coelenterazine
EDTA
Hepes
KCl
X-100
coelenterazine
coelenterazine
coelenterazine
→
2,8-Dibenzyl-3-methoxy-6-phenyl-imidazo[1,2-a]pyrazine
Reagents
None
Solvents
Conditions
Detailed conditions
See reaction.notes.procedure_details.
Procedure
Chemiluminescence of P450 altered coelenterazine or coelenterazine derivatives was determined immediately following addition of 80 microliter of 37.5 mM Hepes pH7.4; 625 mM KCl; 0.125 mM EDTA pH 8.0; 0.25% Triton X-100; 0.125% Mazu; 1.25% Glycerol; 0.25 mg/mL gelatin to the P450 reactions. Renilla luciferase (1.2 ng/mL) (purchased from Chemicon) was added for detection of bioluminescence of P450 altered coelenterazine or coelenterazine derivatives.