Reaction #4988

ord-fef06120f7244b028a882a59eab365e0

Reaction equation

Cn1c(=O)c2[nH]c(CCCN)nc2n(C)c1=O
8-(3-aminopropyl)-theophylline
→
Cn1c(=O)c2[nH]cnc2n(C)c1=O
theophylline

Reactants

Cn1c(=O)c2[nH]c(CCCN)nc2n(C)c1=O
8-(3-aminopropyl)-theophylline
—
N-hydroxysuccinimide ester

Reagents

None

Solvents

O=C([O-])[O-]
carbonate

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    Otherdextran surface (according to Example I.3.2) overnight
  2. 2
    Otherat 25° C.
  3. 3
    Washwhereupon the surface was washed with water

Procedure

A solution of 5 mM 8-(3-aminopropyl)-theophylline (R.C. Boguslaski et al., 1980) in 0.1 M carbonate buffer, pH 8.0, was incubated with an N-hydroxysuccinimide ester activated dextran surface (according to Example I.3.2) overnight at 25° C., whereupon the surface was washed with water.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05242828uspto-grants-1993_09