Reaction #492850

ord-e8dc61f2a583462c83b2582574c4ed4b

Reaction equation

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP
[Cl-].[Cl-].[Mg+2]
MgCl2
[Cl-].[K+]
KCl
CC(=O)C(=O)[O-]
pyruvate
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
ascorbic acid
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvic acid
→
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose

Reactants

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP
[Cl-].[Cl-].[Mg+2]
MgCl2
[Cl-].[K+]
KCl
CC(=O)C(=O)[O-]
pyruvate
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
ascorbic acid
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvic acid

Reagents

None

Solvents

O=S(=O)(O)CCN1CCOCC1
2-morpholinoethanesulfonic acid

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

In 10.0 mM 2-morpholinoethanesulfonic acid (MES) buffer, 17.6 mM MgCl2, 17.6 mM KCl, 175.7 mM phosphoenolpyruvic acid (PEP), 17.6 mM ATP, 123 U/mL pyruvate kinase (PK), 97 U/mL hexokinase, and 20 U/mL ascorbic acid oxidase were dissolved in this composition, and the pH was adjusted to 7.0 to obtain a glucose converting reagent.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08753832B2uspto-grants-2014_06