Reaction #47727

ord-16628f9ac2414b83af4105f0af2fafd5

Reaction equation

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP
[Cl-].[Cl-].[Mg+2]
MgCl2
Cl.NC(CO)(CO)CO
Tris-HCl
→
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ADP

Reactants

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP
—
reaction mixture
—
polyphosphate
[Cl-].[Cl-].[Mg+2]
MgCl2
Cl.NC(CO)(CO)CO
Tris-HCl
—
reaction mixture

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    Otherwere prepared

Procedure

Next, 50 μl of a reaction mixture containing 0.16 μg of the apyrase-treated PPK-ADK, 10 μM AMP, 400 μM polyphosphate, 8 mM MgCl2, and 60 mM Tris-HCl (pH 7.4) were prepared. Then, 5 μl of the reaction mixture were sampled and mixed with 40 μl of an ATP bioluminescence assay reagent (Roche), and luminescence was measured immediately by using a multiplate luminometer (ARVO, Wallac).

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07745160B2uspto-grants-2010_06