Reaction #438706

ord-5a4f5e384ffe4cb687776591f97d51e1

Reaction equation

CCCCC[C@@H](/C=C/C=C\CCCCCCCC(=O)O)OO
13-HPODE
→
CCCCC/C=C\C/C=C\CCCCCCCC(=O)O
linoleic acid

Reactants

CCCCC[C@@H](/C=C/C=C\CCCCCCCC(=O)O)OO
13-HPODE
—
lipid hydroperoxide

Reagents

None

Solvents

OB(O)O.OB(O)O.OB(O)O.[Na+].[Na+].[O-]B([O-])O
di-sodium tetraborate

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

13-HPODE, which is a lipid hydroperoxide was produced by the peroxidation of linoleic acid by soybean lipoxygenase-1, in 100 mM di-sodium tetraborate buffer at pH 9.4. After purification by HPLC, the absorption spectrum of 13-HPODE (80 μM) was obtained (FIG. 31). This shows a peak at 234 nm where the conjugated diene absorbs.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US06743638B1uspto-grants-2004_06