Reaction #368013

ord-c79d5f22457f4288b39dc3404c84ed1f

Reaction equation

[Na+].[OH-]
sodium hydroxide
[Cl-].[Na+]
sodium chloride
[Cl-].[K+]
potassium chloride
O=P([O-])([O-])[O-].[Na+].[Na+].[Na+]
sodium phosphate
→
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
Phosphate buffered saline

Reactants

[Na+].[OH-]
sodium hydroxide
[Cl-].[Na+]
sodium chloride
[Cl-].[K+]
potassium chloride
O=P([O-])([O-])[O-].[Na+].[Na+].[Na+]
sodium phosphate

Reagents

None

Solvents

O
water

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

Phosphate buffered saline (PBS) was prepared by dissolving sodium chloride (0.14M), potassium chloride (2.7 mM), and dibasic sodium phosphate (6.5 mM) in purified water The pH of the solution was adjusted to 7.4±0.2 with concentrated sodium hydroxide.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US04591557uspto-grants-1986_05