Reaction #314696

ord-0ef64fae5d6d4389b6f4873fb004e250

Reaction equation

O=P([O-])(O)O.[K+]
KH2PO4
[Cl-].[Na+]
NaCl
[Cl-].[K+]
KCl
O[C@H](CS)[C@H](O)CS
DTT
→
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
Phosphate Buffered Saline

Reactants

O=P([O-])(O)O.[K+]
KH2PO4
[Cl-].[Na+]
NaCl
[Cl-].[K+]
KCl
O[C@H](CS)[C@H](O)CS
DTT
—
Na2HPO4

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

137 Mm NaCl, 2.6 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4; 1 mM DTT; 1× protease inhibitor cocktail

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07560546B2uspto-grants-2009_07