Reaction #310525
ord-e88ee83601aa49e4a59a7ec3e76ed946
Reactants
Reagents
Solvents
Conditions
Workup
- 1Otherto have been consumed within 24 h
Procedure
Rebaudioside C, 3-sulfopropyl ester, potassium salt prepared as in Part B. was incubated anaerobically for three days at 37° C. with 5% fresh rat cecal contents at concentrations of 0.25, 0.5, and 1.0 mg/ml, in sterile Krebs-Ringer 0.25 M phosphate buffer (pH 7.4) containing 0.25 mg/ml dithiothreitol and 0.25 mg/ml α-D-glucose. TLC [silica gel F-254; CHCl3 :CH3OH:H2O (15:10:2)] and HPLC [30 cm C-18 on μ-Bondapak; 15 min linear gradient of 10-40% CH3CN in 0.005 M KH2PO4 (pH 3.45); 200 nm] analysis showed all the rebaudioside C, 4-sulfopropyl ester (Rf =0.42; tR =14.0 min) to have been consumed within 24 h to yield apparently only the sulfopropyl ester of steviol, (Rf =0.63; tR =16.3 min). No steviol (Rf =0.95; tR =31.3 min) was detected. After 3 days the bacterial cells were sedimented by centrifugation. The sediment was extracted (THF), as was the lyophilized supernatant. HPLC analysis for steviol of the two THF extracts showed none to be detectable. With a detection limit of 0.05 μg, as little as 0.03 and 0.13 percent degradation to steviol could have been detected for the sediment and supernatant fractions, respectively.