Reaction #2831

Reaction equation

1-hydroxybenzotriazole

1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride

morpholine

compound ( [5]-(53)-278 )

4-methyl-3-nitrobenzoic acid

→

crystals

4-methyl-3-nitrobenzoic acid morpholide

Solvents

triethylamine

water

DMF

Conditions

Workup

1. 1
   Otherto obtain a homogeneous solution
2. 2
   ConcentrationThe solution was concentrated
3. 3
   workup.DISSOLUTIONThe residue was dissolved in chloroform
4. 4
   Washthe solution was washed with 1N HCl aqueous solution and saturated brine
5. 5
   DryingThe organic layer was dried over anhydrous sodium sulfate
6. 6
   Concentrationconcentrated

Procedure

The compound ([5]-(53)-278) (6.5272 g) prepared in Example 72 was dissolved in anhydrous DMF (98 ml). Then, 1-hydroxybenzotriazole (6.3297 g), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (8.9795 g), triethylamine (6.5 ml), and morpholine (4.1 ml) were added to the solution. The mixture was stirred at room temperature for 4 hours. A small amount of water was added to the resulting yellowish orange solution including the white salt to obtain a homogeneous solution. The solution was concentrated. The residue was dissolved in chloroform, and the solution was washed with 1N HCl aqueous solution and saturated brine. The organic layer was dried over anhydrous sodium sulfate, and concentrated to obtain yellowish brown crystals (8.9903 g). The product was recrystallized from n-hexane/ethyl acetate to obtain the above-captioned compound ([5]-(54)-278) (7.8455 g) as light yellow crystals.

Source