Reaction #2467954

Reaction equation

(2S,3R)-methyl-2-benzamidomethyl-3-hydroxybutyrate

(2R,3R)-methyl-2-benzamidomethyl-3-hydroxybutyrate

→

Methyl-2-benzamidomethyl-3-oxobutyrate

Conditions

Workup

1. 1
   workup.ADDITIONSimilarly, an ADH-LK variant with SEQ ID No.94 containing 10 mutations
2. 2
   Othergave primarily the (2R,3R) stereoisomer

Procedure

The ADH-LK variant with SEQ ID No. 90 was used for further evolution of an efficient KRED for production of (2S,3R)-methyl-2-benzamidomethyl-3-hydroxybutyrate. Similarly, an ADH-LK variant with SEQ ID No.94 containing 10 mutations compared to ADH-LK which gave primarily the (2R,3R) stereoisomer, was used for further evolution of an efficient KRED for production of (2R,3R)-methyl-2-benzamidomethyl-3-hydroxybutyrate.

Source