Reaction #2446102

ord-73a2724085a0476c89499840040c926d

Reaction equation

O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O
EDTA
CC(=O)[O-].[Na+]
sodium acetate
→
CC(=O)[O-].CCO.O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O.[Na+]
Ethanol EDTA Sodium Acetate

Reactants

O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O
EDTA
CC(=O)[O-].[Na+]
sodium acetate

Reagents

None

Solvents

CCO
ethanol

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    OtherThe plate is sealed
  2. 2
    workup.ADDITIONmixed
  3. 3
    WashThen the precipitated DNA is washed with 75% ethanol for 3 times

Procedure

2 μL of 125 mM EDTA and 2 μL of 3 M sodium acetate are added to each well. And then 50 μL 100% ethanol is added to each well. The plate is sealed and mixed by inverting 4 times. The plate is incubated at room temperature for 15 minutes. Then the precipitated DNA is washed with 75% ethanol for 3 times.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08685647B2uspto-grants-2014_04