Reaction #2320137

ord-78472cffd067490d93099f8fe510eeb4

Reaction equation

O=C[C@H](O)[C@@H](O)[C@H](O[C@H]1O[C@H](CO[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@H](O)[C@H]1O)[C@H](O)CO
panose
→
OC[C@H]1O[C@H](OC[C@H]2OC(O)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
Isomaltose

Reactants

O=C[C@H](O)[C@@H](O)[C@H](O[C@H]1O[C@H](CO[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@H](O)[C@H]1O)[C@H](O)CO
panose
—
α-isomaltosylmaltose
—
saccharide

Reagents

None

Solvents

O
water

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    workup.ADDITIONα-isomaltosyltriose, α-isomaltosyltetraose, or cyclotetrasaccharide, were added 100 units/g solid of an isomaltodextranase specimen
  2. 2
    Otherobtained by the method in Experiment 15
  3. 3
    Otherto react at 40° C.
  4. 4
    workup.WAITkept at 100° C. for 20 min
  5. 5
    Other80° C., inner column temperature

Procedure

To an aqueous solution having a final solid concentration of 0.2% (w/v) of panose, α-isomaltosylmaltose, α-isomaltosyltriose, α-isomaltosyltetraose, or cyclotetrasaccharide, were added 100 units/g solid of an isomaltodextranase specimen, obtained by the method in Experiment 15, except for using 100 or 3,000 units of the specimen for the aqueous solution of cyclotetrasaccharide, allowed to react at 40° C. and pH 5.5 for 24 hours, and kept at 100° C. for 20 min to suspend the enzymatic reactions. The saccharide composition for each reaction mixture was determined on HPLC. The conditions used in HPLC were: Column, “MCIGEL CK04SS” comercialized by Mitsubishi Chemical Industries, Ltd., Tokyo, Japan; 80° C., inner column temperature; 0.5 ml/min, a flow rate of water as an eluent; and detection, “RI-8012”, a diffraction refractometer commercialized by Tosoh Corporation, Tokyo, Japan. The results are in Table 18.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07709230B2uspto-grants-2010_05