Reaction #2320136

Reaction equation

maltose

maltotriose

→

isomaltosyl maltose

maltose

Solvents

acetate

Conditions

Workup

1. 1
   Otherto give a concentration of 2% (w/v) for a substrate solution
2. 2
   Otherat 35° C.
3. 3
   Otherfor 60 min

Procedure

The activities of these enzymes were assayed as follows: The α-isomaltosylglucosaccharide-forming enzyme of the present invention was assayed for enzymatic activity by dissolving maltotriose in 100 mM acetate buffer (pH 6.0) to give a concentration of 2% (w/v) for a substrate solution, adding a 0.5 ml of an enzyme solution to a 0.5 ml of the substrate solution, enzymatically reacting the mixture solution at 35° C. for 60 min, suspending the reaction mixture by heating at 100° C. for 10 min, and quantifying maltose, among the isomaltosyl maltose and maltose formed in the reaction mixture, by high-performance liquid chromatography (abbreviated as “HPLC” hereinafter). HPLC was carried out using “YMC PACK ODS-AQ303 column” commercialized by YMC Co., Ltd., Tokyo, Japan, at a column temperature of 40° C. and a flow rate of 0.5 ml/min of water; and using “RI-8012”, a differential refractometer commercialized by Tosoh Corporation, Tokyo, Japan. One unit activity of the α-isomaltosylglucosaccharide-forming enzyme is defined as the enzyme amount that forms one micromole of maltose per minute under the above enzymatic reaction conditions.

Source