Reaction #2307980

Reaction equation

dicyclohexylcarbodiimide

N-hydroxysuccinimide

N,N-biscarboxymethyl-L-lysine

→

Nitrilotriacetic Acid

Conditions

Workup

1. 1
   Washwere washed twice with 1 ml of acetonitrile (MeCN)
2. 2
   WashWashing
3. 3
   workup.STIRRINGshaken at room temperature for three hours
4. 4
   Washby washing once with 1 ml of acetonitrile and twice with 1 ml of 10 mM phosphate buffer (pH 7.0)

Procedure

10 mg of carboxy-modified carriers were washed twice with 1 ml of acetonitrile (MeCN) and then taken up in 1 ml of MeCN. To this were added 10 mmol of dicyclohexylcarbodiimide and 10 μmol of N-hydroxysuccinimide. This was followed by shaking at room temperature for two hours. Washing was then carried out once with 1 ml of cyclohexane and once with 1 ml of MeCN. The particles were then taken up in 1 ml of MeCN. 4 μmol of N,N-biscarboxymethyl-L-lysine were added thereto and shaken at room temperature for three hours. This was followed by washing once with 1 ml of acetonitrile and twice with 1 ml of 10 mM phosphate buffer (pH 7.0).

Source