Reaction #2307971

Reaction equation

Glucose

sodium carbonate

succinic acid

→

malic acid

Conditions

Workup

1. 1
   OtherThe coryneform bacterium cells prepared after culturing
2. 2
   workup.ADDITIONwere added to 500 ml of the reaction stock solution in a reaction container under the nitrogen gas atmosphere
3. 3
   Otherproducing an organic compound
4. 4
   Otherafter the initiation of the reaction
5. 5
   Temperatureby maintaining the oxidation-reduction potential at −400 mV
6. 6
   OtherAfter the reaction for 3 hours

Procedure

The coryneform bacterium cells prepared after culturing were added to 500 ml of the reaction stock solution in a reaction container under the nitrogen gas atmosphere. Glucose 200 mM and sodium carbonate 200 mM were added, and the reaction temperature was maintained at 33° C. to perform a reaction producing an organic compound. An oxidation-reduction potential was initially −200 mV, but was reduced immediately after the initiation of the reaction, and the reaction was continued by maintaining the oxidation-reduction potential at −400 mV. After the reaction for 3 hours, the reaction medium solution was analyzed by liquid chromatography, indicating that succinic acid 163 mM (19.2 g/L) and malic acid 5 mM (0.67 g/L) were produced. Lactic acid was not detected.

Source