Reaction #2200575

Reaction equation

Hepes

→

ethanol Hepes

Yield 50.0%

Solvents

ethanol

Conditions

Workup

1. 1
   Otherincubation overnight
2. 2
   Otherat 4° C

Procedure

Gilmour, Plant Physiology 2000, 124, 1854-1865 extracts sugar from lyophilized Arabidopsis leaves in 80% ethanol following incubation for 15 minutes at 80° C. and incubation overnight at 4° C. Strand, Plant Physiology 1999, 119, 1387-1397 extracts soluble sugars and starch twice in succession, likewise at 80° C. and for 30 minutes and in 80% ethanol with Hepes, pH 7.5. The material is then reextracted twice at this high temperature to improve the result of the extraction, once with 50% ethanol/Hepes, pH 7.5, and once with Hepes, pH 7.5.

Source