Reaction #2146721

ord-446bf358db37414e8737611d2119db3a

Reaction equation

[Cl-].[Na+]
sodium chloride
O=P([O-])(O)O.[K+]
potassium dihydrogen phosphate
[Ca+2].[Cl-].[Cl-]
calcium chloride
[Cl-].[Na+]
sodium chloride
[Cl-].[K+]
potassium chloride
[Cl-].[K+]
potassium chloride
O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate
[Ca+2].[Cl-].[Cl-]
calcium chloride
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
D-glucose
O=P([O-])(O)O.[K+]
potassium dihydrogen phosphate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
D-glucose
O=S(=O)(O)CCCN1CCOCC1.[Na+].[OH-]
MOPS NaOH
O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate
O=C(O)CNC(CO)(CO)CO
tricine
→
CN1CCCC1c1cccnc1
Nicotine

Reactants

[Cl-].[Na+]
sodium chloride
O=P([O-])(O)O.[K+]
potassium dihydrogen phosphate
[Ca+2].[Cl-].[Cl-]
calcium chloride
[Cl-].[Na+]
sodium chloride
[Cl-].[K+]
potassium chloride
[Cl-].[K+]
potassium chloride
O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate
[Ca+2].[Cl-].[Cl-]
calcium chloride
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
D-glucose
O=P([O-])(O)O.[K+]
potassium dihydrogen phosphate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
D-glucose
O=S(=O)(O)CCCN1CCOCC1.[Na+].[OH-]
MOPS NaOH
O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate
O=C(O)CNC(CO)(CO)CO
tricine

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

The activity at pH 8.0 was determined using a buffer comprising 125 mM sodium chloride, 4.8 mM potassium chloride, 5.6 mM D-glucose, 1.2 mM calcium chloride, 1.2 mM potassium dihydrogen phosphate, 1.2 mM magnesium sulfate, and 25 mM tricine. The activity at pH 7.0 was further determined using a buffer comprising 125 mM sodium chloride, 4.8 mM potassium chloride, 5.6 mM D-glucose, 1.2 mM calcium chloride, 1.2 mM potassium dihydrogen phosphate, 1.2 mM magnesium sulfate, and 25 mM MOPS-NaOH.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08278032B2uspto-grants-2012_10